Browsing by Author "Cruzado-Bravo, Melina L.M."

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Detection of Listeria innocua in the dairy processing chain: resistance to antibiotics and essential oils.
(Sociedade Brasileira de Ciencia e Tecnologia de Alimentos, 2022-06) Cardoso da Rosa, Mayara; Iacuzio, Raiza; Rueda Barbosa, Giovana; Lisboa Pereira, Rodrigo De Castro; Cruzado-Bravo, Melina L.M.; Mores Rall, Vera Lúcia; Vallim, Deyse Christina; Cirone Silva, Nathália Cristina
Listeria monocytogenes is a food pathogen responsible for many foodborne disease outbreaks. Listeria innocua is similar to L. monocytogenes, could be considered biologically similar to L. monocytogenes and could be used to predict its behavior. The aims of this work were to isolate Listeria spp. in dairy processing plants, perform genotypic characterization of virulence, evaluate the antimicrobial resistance profile, and verify the sensitivity of the strains to essential oils. Sampling was held in six dairy processing plants in the region of Campinas, Brazil. Seventeen Listeria innocua strains were isolated, all negative for virulence genes inlA, inlB, inlC, inlJ, actA, plcA, and plcB. The strains were resistant to kanamycin (5.9%), penicillin (11.8%), ampicillin (11.8%), cefoxitin (58.8%), clindamycin (76.50%) and oxacillin (100%). The susceptibility of the strains to commercial essential oils (Rosemarinus officinalis, Cymbopogon citratus, Eucalyptus citirodora, Mentha piperita, Piper nigrum, Vetiveria zizanioides, Cymbopogon nardus, Cymbopogon martinii and Pogostemon cablin) was evaluated by Minimum Inhibitory Concentration (MIC) test. The results showed Cymbopogon citratus, Cymbopogon martini, Cymbopogon nardus and Mentha piperita oils were most efficient. The resistance of Listeria innocua isolated in dairy processing plants is worrisome, and the use of essential oils could be an alternative for development of new drugs and products.
Effect of salt reduction, mixture of salt with animal fat, and salt particle sizes on instrumental texture, yield properties and sensory characteristics of burgers.
(Springer Nature, 2024-10) Coronado, Agataliz; Tocto, Kori; Tello Célis, Fernando; Ruiz, Roger; Vásquez, Jessy; Chiroque, Grisel Violeta; Cruzado-Bravo, Melina L.M.; Saldaña, Erick; Rios‑Mera, Juan D.
This study aimed to investigate the impact of reduction of salt content (from 1.5 to 0.75%), the technique of mixing half of the salt content with animal fat, and the salt particle size on the instrumental texture, cooking losses, diameter reduction, overall liking, and sensory characteristics of burgers. The results showed that regardless of the types of micronized salt (MS<250 µm) incorporation (directly into the meat or the mixture of half of the MS with the meat and the other half with the fat), salt reduction decreased the salty perception and the instrumental hardness and chewiness. Thus, the mixture of MS with fat does not present sensory improvements nor overcome the texture effects of salt reduction. In a second experiment, the effect of different particle sizes (from <177 µm to 1 mm) was evaluated, where it was observed that salt with particle size <177 µm decreased the burgers’ hardness, cooking losses, and diameter reduction. The salt particle sizes did not cause sensory changes in the burgers, and in both experiments, the overall liking was greater than 7 points on the 9-point hedonic scale. Salt<177 µm could be a good option for reducing salt in burgers and possibly in other meat products.
Exploring Pijuayo (Bactris gasipaes) Pulp and Peel Flours as Fat Replacers in Burgers.
(Multidisciplinary Digital Publishing Institute, 2024-05) Llatas, Alex Y.; Guzmán, Heiner; Tello Célis, Fernando; Ruiz, Roger; Vásquez, Jessy; Chiroque, Grisel Violeta; Mayta-Hancco, Jhony; Cruzado-Bravo, Melina L.M.; Arteaga, Hubert; Saldaña, Erick
Meat products are known for their lipid profile rich in saturated fats and cholesterol, and also for the formation of oxidation compounds; therefore, a reduction in animal fat may result in a product less harmful to health. Pijuayo is an Amazon fruit known for its nutritional properties, such as its fiber and lipid content. For these reasons, it is an attractive fruit to replace animal fat in meat products. The present work used pijuayo pulp and peel flours to partially replace animal fat in beef-based burgers at 25% and 50% levels, considering sensory and physicochemical outcomes evaluated by Principal Component Analysis (PCA), Correspondence Analysis (CA) and Multiple Factor Analysis (MFA). Pijuayo flour affected the physicochemical characteristics evaluated by PCA, where the samples with greater fat replacement were characterized by a high carbohydrate content and instrumental yellowness. The minimal fat replacement did not abruptly affect the PCA’s instrumental texture and color, proximal composition, yield properties, and lipid oxidation. The overall liking was greater in the 25% fat reduction treatments, even greater than the control, in which positive sensory attributes for liking were highlighted for those treatments. A small segment of consumers (11% of total consumers) preferred the treatment with greater replacement of fat with pijuayo peel flour, which these consumers tended to characterize as seasoned. However, this treatment had the lowest liking. The MFA showed that the sensory characteristics tender and tasty were strongly correlated with overall liking and were highlighted in the samples of 25% fat reduction, suggesting that the pijuayo improves the tenderness and flavor of reduced-fat burgers. Other inclusion levels between 25% and 50% of fat replacement could be explored, and optimization studies are needed. In addition, the sensory characteristics and flavor-enhancing compounds of the fruit, as well as the nutritional aspects of the inclusion of pijuayo, should be studied, such as the fatty acid profile. These characteristics will be informative to explore pijuayo as a fat replacer at a pilot scale and industrial scale.
Incorporation of nisin and oregano essential oil in cow’s milk to improve the quality of fresh cheese.
(Universidad Nacional de Trujillo, 2022-12) Cama-Curasi, John Víctor; Saldaña, Erick; Cruzado-Bravo, Melina L.M.; S. Ambrosio, Carmen M.; Mayta-Hancco, Jhony
Firstly, this study aimed to determine the effect of adding nisin (0, 250 and 500 IU mL-1) and oregano essential oil (OEO) (0, 0.025% and 0.05%), alone or in combination, on Staphylococcus aureus load in cow’s milk. Next, it was evaluated the sensory, physicochemical and microbiological quality of fresh cheese (FC) manufactured with milk added of nisin + OEO (C1) or nisin alone (C2) compared to a control cheese (C0, milk without the addition of nisin or OEO). Results showed that adding nisin effectively reduced the S. aureus count in milk in a similar manner to adding way the combination of nisin + OEO compared to the control. FC quality evaluation results revealed that C1 and C2 did not alter the physicochemical quality of FC (pH, fat and total solids content) during storage (0-8 days) compared to the control. Furthermore, C1 and C2 reduced in a similar manner aerobic mesophilic bacteria and Enterobacteriaceae count in FC compared to the control during storage. Sensory evaluation showed that consumers did not value FC added of nisin + OEO (C1) due to its intense aroma of oregano, rating it with the lowest global quality.