Browsing by Author "Guelac-Santillan, Marly"

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Effect of arbuscular mycorrhiza on germination and initial growth of Cinchona officinalis L. (Rubiaceae).
(Taylor and Francis, 2022-09) Fernandez-Zarate, Franklin Hitler; Huaccha-Castillo, Annick Estefany; Quiñones Huatangari, Lenin; Vaca-Marquina, Segundo Primitivo; Sánchez-Santillan, Tito; Morales-Rojas, Eli; Seminario-Cunya, Alejandro; Guelac-Santillan, Marly; Barturén Vega, Luci Magali; Coronel-Bustamante, David
Cinchona officinalis, known locally as cascarilla or cinchona, is a plant species native to South America. It was used as a source of quinine to combat malaria in the 17th century. The species is threatened by various anthropogenic activities. Further, the propagation of the species depends on seed dispersal and its germination capacity. Therefore, it is necessary to conserve and propagate this species. Because C. officinalis seeds have a low germination capacity, we determined the effect of arbuscular mycorrhizae (AM) on their germination and growth. A randomized design was employed with two treatments, one treated with mycorrhizae (CM) and another without mycorrhizae (SM). For each treatment, three replicates of 100 seeds were used. Germination, growth, and fungal characteristics were evaluated. In germination parameters, the CM treatment showed better performance, but the improvement was statistically insignificant. However, the application of AM significantly improved seedling height (cm), root length (cm), leaf area (cm2), and root number by 53.52, 28.72, 29.73, and 61.66%, respectively. Likewise, mycorrhization intensity (%), mycorrhization frequency (%), and extraradical mycelium length (cm) in the CM treatment were 37.13, 3.44, and 174.97% higher compared to the SM treatment, respectively. Therefore, the use of AM fungi proves to be advantageous in the propagation of C. officinalis, and these results provide a basis for the largescale and sustainable propagation of this species.
Effect of arbuscular mycorrhizae on the growth of Cinchona officinalis L. (rubiaceae) in nursery.
(Taylor and Francis, 2024-04) Fernández-Zárate, Franklin Hitler; Huaccha-Castillo, Annick Estefany; Quiñones Huatangari, Lenin; Vaca-Marquina, Segundo Primitivo; Sánchez-Santillan, Tito; Guelac-Santillan, Marly; Seminario-Cunya, Alejandro
Cinchona officinalis, commonly called cascarilla or quina, has medicinal value; and is on Peru’s national coat of arms representing its plant wealth (flora), however, it is threatened by anthropogenic activities. This study aimed to determine the effect of the commercial product Myco Grow on the growth of C. officinalis in nursery. A randomized design was used with two treatments, one with Myco Grow application (WM) and the other without incorporating this commercial product (NM). Each treatment had three replicates consisting of 30 plants each. Monthly evaluations were performed, during which the number of dead plants, plant height, and plant diameter were recorded. Additionally, at the end of the study, the anhydrous weight of leaves, stems, and roots; leaf area; mycorrhizal frequency; mycorrhizal colonization index; and the length of extra-radicular mycelia were determined. The WM treatment achieved 36.6% lower mortality, 38.01% greater height, and 48.52% greater diameter than the NM treatment. Additionally, inoculation with arbuscular mycorrhizae (AM) improved the anhydrous weights of the leaves, stems, roots, and leaf area by 84.31%, 84.28%, 70.85%, and 76.91%, respectively. Regarding the three fungal variables analyzed for the WM treatment; mycorrhizal frequency was 87%, AM application led to a mycorrhizal intensity of 7.7% and an extra-radicular mycelium length of 90.3cm. This study confirmed that AM positively influences the growth of C. officinalis in the nursery and can be used to sustainably produce this species on a large scale.