Browsing by Author "Vaca-Marquina, Segundo Primitivo"

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Effect of arbuscular mycorrhiza on germination and initial growth of Cinchona officinalis L. (Rubiaceae).
(Taylor and Francis, 2022-09) Fernandez-Zarate, Franklin Hitler; Huaccha-Castillo, Annick Estefany; Quiñones Huatangari, Lenin; Vaca-Marquina, Segundo Primitivo; Sánchez-Santillan, Tito; Morales-Rojas, Eli; Seminario-Cunya, Alejandro; Guelac-Santillan, Marly; Barturén Vega, Luci Magali; Coronel-Bustamante, David
Cinchona officinalis, known locally as cascarilla or cinchona, is a plant species native to South America. It was used as a source of quinine to combat malaria in the 17th century. The species is threatened by various anthropogenic activities. Further, the propagation of the species depends on seed dispersal and its germination capacity. Therefore, it is necessary to conserve and propagate this species. Because C. officinalis seeds have a low germination capacity, we determined the effect of arbuscular mycorrhizae (AM) on their germination and growth. A randomized design was employed with two treatments, one treated with mycorrhizae (CM) and another without mycorrhizae (SM). For each treatment, three replicates of 100 seeds were used. Germination, growth, and fungal characteristics were evaluated. In germination parameters, the CM treatment showed better performance, but the improvement was statistically insignificant. However, the application of AM significantly improved seedling height (cm), root length (cm), leaf area (cm2), and root number by 53.52, 28.72, 29.73, and 61.66%, respectively. Likewise, mycorrhization intensity (%), mycorrhization frequency (%), and extraradical mycelium length (cm) in the CM treatment were 37.13, 3.44, and 174.97% higher compared to the SM treatment, respectively. Therefore, the use of AM fungi proves to be advantageous in the propagation of C. officinalis, and these results provide a basis for the largescale and sustainable propagation of this species.
Effect of arbuscular mycorrhizae on the growth of Cinchona officinalis L. (rubiaceae) in nursery.
(Taylor and Francis, 2024-04) Fernández-Zárate, Franklin Hitler; Huaccha-Castillo, Annick Estefany; Quiñones Huatangari, Lenin; Vaca-Marquina, Segundo Primitivo; Sánchez-Santillan, Tito; Guelac-Santillan, Marly; Seminario-Cunya, Alejandro
Cinchona officinalis, commonly called cascarilla or quina, has medicinal value; and is on Peru’s national coat of arms representing its plant wealth (flora), however, it is threatened by anthropogenic activities. This study aimed to determine the effect of the commercial product Myco Grow on the growth of C. officinalis in nursery. A randomized design was used with two treatments, one with Myco Grow application (WM) and the other without incorporating this commercial product (NM). Each treatment had three replicates consisting of 30 plants each. Monthly evaluations were performed, during which the number of dead plants, plant height, and plant diameter were recorded. Additionally, at the end of the study, the anhydrous weight of leaves, stems, and roots; leaf area; mycorrhizal frequency; mycorrhizal colonization index; and the length of extra-radicular mycelia were determined. The WM treatment achieved 36.6% lower mortality, 38.01% greater height, and 48.52% greater diameter than the NM treatment. Additionally, inoculation with arbuscular mycorrhizae (AM) improved the anhydrous weights of the leaves, stems, roots, and leaf area by 84.31%, 84.28%, 70.85%, and 76.91%, respectively. Regarding the three fungal variables analyzed for the WM treatment; mycorrhizal frequency was 87%, AM application led to a mycorrhizal intensity of 7.7% and an extra-radicular mycelium length of 90.3cm. This study confirmed that AM positively influences the growth of C. officinalis in the nursery and can be used to sustainably produce this species on a large scale.
Effect of synthetic fertilization dose on the diameter increase, height and mortality of Cinchona officinalis L. (Rubiaceae)
(Taylor and Francis, 2024-04) Fernandez-Zarate, Franklin Hitler; Huaccha-Castillo, Annick Estefany; Quiñones Huatangari, Lenin; Vaca-Marquina, Segundo Primitivo; Goñas, Malluri; Milla Pino, Manuel Emilio; Seminario-Cunya, Alejandro
Cinchona officinalis, is a South American tree species, commonly used for medicine, and is currently threatened by agricultural expansion and cattle ranching. The objective was to determine the effect of chemical fertilization on the nursery growth to increase growth potential and survival of C. officinalis. A completely randomized design with six treatments and three replicates was used; 20 C. officinalis plants were used per replicate. Two months after transplanting the C. officinalis seedlings to the polyethylene bags, inorganic fertilizer (YaraMila® HYDRAN) was applied. Monthly evaluations were carried out and the number of dead plants, plant height, diameter and number of leaves were recorded. The highest mortality rate was recorded when fertilizer was applied (73%) while in the non-fertilized treatment mortality reached 36%. Regarding the increase in height, diameter and number of leaves in all cases, the best results were obtained in the fertilized treatments, exceeding by 85, 70 and 17% (respectively) those obtained in the treatment to which fertilization was not applied. This study shows the effects that the application of fertilizers to C. officinalis plants at the nursery level can have on growth and mortality variables, the results suggest the use of this product for a sustainable and large-scale production of this species taking into consideration the appropriate dosage.
Non-destructive estimation of leaf area and leaf weight of Cinchona officinalis L. (Rubiaceae) based on linear models.
(Taylor and Francis, 2023-06) Huaccha-Castillo, Annick Estefany; Fernandez-Zarate, Franklin Hitler; Pérez-Delgado, Luis Jhoseph; Tantalean-Osores, Karla Saith; Vaca-Marquina, Segundo Primitivo; Sánchez-Santillan, Tito; Morales-Rojas, Eli; Seminario-Cunya, Alejandro; Quiñones Huatangari, Lenin
Non-destructive methods that accurately estimate leaf area (LA) and leaf weight (LW) are simple and inexpensive, and represent powerful tools in the development of physiological and agronomic research. The objective of this research is to generate mathematical models for estimating the LA and LW of Cinchona officinalis leaves. A total of 220 leaves were collected from C. officinalis plants 10 months after transplantation. Each leaf was measured for length, width, weight, and leaf area. Data for 80% of leaves were used to form the training set, and data for the remaining 20% were used as the validation set. The training set was used for model fit and choice, whereas the validation set al.lowed assessment of the of the model’s predictive ability. The LA and LW were modeled using seven linear regression models based on the length (L) and width (Wi) of leaves. In addition, the models were assessed based on calculation of the following statistics: goodness of fit (R2), root mean squared error (RMSE), Akaike’s information criterion (AIC), and the deviation between the regression line of the observed versus expected values and the reference line, determined by the area between these lines (ABL). For LA estimation, the model LA ¼ 11.521(Wi) 21.422 (R2 ¼ 0.96, RMSE ¼ 28.16, AIC¼ 3.48, and ABL¼ 140.34) was chosen, while for LW determination, LW ¼ 0.2419(Wi) 0.4936 (R2 ¼ 0.93, RMSE ¼ 0.56, AIC ¼ 37.36, and ABL¼ 0.03) was selected. Finally, the LA and LW of C. officinalis could be estimated through linear regression involving leaf width, proving to be a simple and accurate tool.